Coding
Part:BBa_K3332026:Design
Designed by: Zhichun Yang Group: iGEM20_XMU-China (2020-10-17)
phnJ_mut40&16
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 549
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 597
Illegal AgeI site found at 268
Illegal AgeI site found at 775 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Put the one with best activity in the final circuit with phn series, RNAi circuits with best activity and kill switch.
Source
Point mutation